Abstract

Autoimmune thyroid disease is an immune problem whose incidence has increased in the past decades among individuals of all ages. Five ml of blood was collected from 60 patients from children with thyroiditis disease and 60 uninfected people for comparisation with average age of the two groups (8.17±1.5 and 9.6±1.2 years)respectively, 2 ml of each sample was used for polymerase chain reaction to detect TLR4-rs1927911-C/T gene polymorphism by amplification-refractory mutation system (ARMS-PCR) and remaining (3ml) for TLR4 Enzyme Linked Immunosorbent Assay and thyroid hormones tests. Our results indicated that C allele of TLR4 (rs1927911) polymorphism was more observed frequently in patients compared to healthy people, at a statistically significant level (P < 0.05). Enzyme Linked Immunosorbent Assay tests also showed that the level of TLR4 in the serum of patients was higher than it was in the serum of healthy people (P=0.001). We conclude from the results of this study that the TLR4-rs1927911-C/T gene polymorphism is associated with autoimmune thyroid disease in among Iraqi children, and may be a cause of the disease and its incidence in both sexes.